Test Information

To locate information on a particular test, click on the relevant letter and follow the trail. eg To find information on sample requirements, test availability and turnaround time for FeLV, click on "F" and then click on the relevant test (FeLV soluble Ag or FeLV IFA).

To download a copy of our lab request form please click link below:

LAB REQUEST FORM


A:G Ratio (albumin:globulin)
Suitable Sample:
  • PC
  • LH
  • EDTA
Unsuitable Sample:
  • CIT
  • FL 
Minimum Sample Size:
  • 2 mL whole blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
  • CP1, 2, 3, 4, 5, 6
  • FP1, 2, 3, 4, 5
  • EP1, 2, 3
  • RP1, 2, 3, 4
  • AP1, 2, 3, 4
Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
Acetylcholinesterase
Suitable Sample:
  • LH
  • Brain
Unsuitable Sample:
  • PC
  • EDTA
Minimum Sample Size:
  • 3ml whole LH blood
  • 50g brain tissue
    Turnaround Time:

    2 weeks

    Request/Test Included in:
    • Request test

     

    Comments:
    • Reduced nonspecific esterase activity in the blood is an indicator of exposure to organophosphates.

     

    Internal Use Only:
    • Send to Ag Dept
    Internal Codes: X03
    Internal Laboratory: Ag Dept
    Internal Test Volume:
    ACTH (Endogenous)
    Suitable Sample:
    • EDTA plasma (plastic tube only)
    • There are published data for this assay method for dogs and horses, but not for other species
    • See Comments for specific collection and storage instructions
    Unsuitable Sample:
    • PC
    • LH
    • SC
    • Samples from species other than canine or equine
    Minimum Sample Size:
    • 1 ml whole blood
    Turnaround Time:
    • 4 hours
    Request/Test Included in:
    • Request Test
    Comments: The information below is also available on this website (ACTH)
    • Preferably fast the animal overnight prior to collection.
    • The animal should not be stressed or excited.
    • Collect a minimum of 1 mL of blood into a plastic EDTA tube.
    • Immediately place the sample in the refrigerator; this is important as ACTH deteriorates rapidly at room temperature.  Do not centrifuge the sample.
    • If possible, keep the sample cool during transit to the laboratory; this is particularly important during the summer months.
    • Samples collected and stored correctly are stable for at least 48 hours.
    REF:Scott-Moncrieff JK, Koshko MA et al. (2003).Validation of a chemiluminescent enzyme immunometric assay for plasma adrenocorticotropic hormone in the dog. Vet Clin Path 23:180-187.
    Internal Use Only:
    • Client Information sheet available (DCI-06)
    • Comment re dogs and horses ONLY added 28-5-08 (MMcC)
    Internal Codes: E07
    Internal Laboratory: VP
    Internal Test Volume: 150 uL plasma
    ACTH Stimulation Test (cortisol x 2)
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • LH
    • EDTA
    • FL
    Minimum Sample Size:
    • 2ml in each PC tube
    Turnaround Time:

    6 hours

    Request/Test Included in:
    • Request test
    Comments:
    • The protocols for the ACTH stimulation test can be found in Client Information Sheets.
    • The cortisol assay moved from the Immulite 2000 to the Cobas Pro in November 2020. Note that results may be affected by methodology and care must be taken when using published reference intervals. Contact the laboratory to speak with a pathologist for assistance in interpreting cortisol results.
    Internal Use Only:
    Internal Codes: E02
    Internal Laboratory: VP
    Internal Test Volume:
    Adenovirus antibody titre
    (Canine Adenovirus Antibody Titre - Infectious Canine Hepatitis)
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • EDTA
    • LH
    • CIT
    • FL
    Minimum Sample Size:
    • 3 mL whole blood

     

    Turnaround Time:
    • Referred test - Up to 4 weeks 
    Request/Test Included in:
    • Request Test

     

    Comments:
    • Samples are sent on Mondays and results are usually returned within two weeks.
    • Can be done as an individual test, or as a combination (distemper, parvovirus, adenovirus).
    Internal Use Only:
    • Canine adenovirus (infectious canine hepatitis) testing is done at Biobest in Scotland.
    • Unless specifically requested, distemper and parvovirus antibody titres requested at the same time as an adenovirus titre are done at Vetpath and only the adenovirus test sent to Biobest.
    Internal Codes:
    • S13B (adenovirus by VN)
    • S15B (combination distemper, parvovirus and adenovirus ONLY IF ALL are going to Biobest)
    • U
    Internal Laboratory: Biobest, Scotland
    Internal Test Volume: 1 ml clear serum
    Albumin
    Suitable Sample:
    • PC
    • LH
    • EDTA
    Unsuitable Sample:
    • FL

     

    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • Request test
    • CP1, 2, 3, 4, 5, 6
    • FP1, 2, 3, 4, 5
    • EP1, 2, 3
    • RP1, 2, 3, 4
    • AP1, 2, 3, 4
    • Liver monitoring panels (all species)
    Comments:
    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume: 25 uL
    Aldosterone
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • EDTA
    • LH
    • CIT
    • FL
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 7 working days

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • Published reference ranges available.
    • Can be measured with renin, however the validity of the renin assay in veterinary species is uncertain.
    • Renin measurement requires EDTA plasma that has been frozen within 1 hour of collection
    Internal Use Only:
    • Added May 2008
    • Think the method is RIA (same as for published ranges but need to verify this with WDP)
    • Aldosterone - 500uL - 1ml serum
    • Renin - 1ml EDTA plasma frozen within 1 hour of collection
    Internal Codes: E31
    Internal Laboratory:
    • WDP
    • Goes to WDP manual biochemistry
    Internal Test Volume: See above
    Allergy Test - Allercept IgE
    Suitable Sample:
    • PC
    • Store at 2-8oC prior to sending to the laboratory
    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 6 mL whole blood (Take enough blood to yield at least 3ml of serum)
    Turnaround Time:
    • 2-3 weeks

     

    Request/Test Included in:
    • Allercept Allergy test (need to differentiate from the Canine ELISA)

     

    Comments:
    • Store sample at 2-8oC before dispatch
    • Sample is to be sent to Vetpath as soon as possible
    • Can be used in dogs, cats and horses
    Internal Use Only:
    Internal Codes: S06
    Internal Laboratory: Gribbles VIC
    Internal Test Volume:
    ALP (Alkaline phosphatase)
    Suitable Sample:
    • PC
    • LH
    Unsuitable Sample:
    • EDTA
    • CIT
    • FL
    Minimum Sample Size:
    • 1.0 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • CP1, 2, 3, 4, 5, 6
    • FP1, 2, 3, 4, 5
    • EP1, 2, 3
    • AP2, 4
    • Liver monitoring panels (all species)
    • Phenobarbitone monitoring panel
    Comments:
    • The corticosteroid isoenzyme is no longer measured.

     

    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume: 25 uL
    Alpha-1 Acid Glycoprotein
    (no longer available)
    Suitable Sample:
    • PC (serum) is preferred
    • Can be run on EDTA and LH plasma or other fluids if required (but these are not suitable for the other components of the FIP screen)
    • The test is feline specific
    Unsuitable Sample:  
    Minimum Sample Size:
    • 1 mL whole blood

     

    Turnaround Time:
    • Monday to Friday on demand
    • Takes 24 hours to run
    Request/Test Included in:
    • FIP Screen
    • Can be requested as an individual test
    Comments:
    • Alpha-1 AGP is an acute phase protein that rises during inflammation.  This test is not specific for FIP, but levels >1.5 g/L have a reported sensitivity of 85% and specificity of 100%,  and efficiency of 90% in separating cats with FIP from cats with clinically similar conditions.

                REF: Vet Record (1997) 141:299-303.
    Internal Use Only:
    • Agar gel immunodiffusion test which takes 24 hours to run
    • Feline specific
    Internal Codes:
    • S28 for individual test
    • S23S for FIP Screen
    • S23SL for FIP Screen for laboratories
    Internal Laboratory: VP
    Internal Test Volume: Alpha-1 AGP alone  5 uL
    ALT (Alanine aminotranferase)
    Suitable Sample:
    • PC
    • LH
    • EDTA
    Unsuitable Sample:
    • FL

     

    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • Request test
    • CP1, 2, 3, 4, 5, 6
    • FP1, 2, 3, 4, 5
    • Canine & feline liver monitoring panels
    • Phenobarbitone monitoring panel
    Comments:
    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume: 30 uL
    Amylase
    Suitable Sample:
    • PC
    • LH
    Unsuitable Sample:
    • EDTA
    • FL
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • Request test
    • CP1, 2, 5, 6
    Comments:
    Internal Use Only: Used to be able to use EDTA plasma, cannot with current method.
    Internal Codes: {Codes(s)}
    Internal Laboratory: VP
    Internal Test Volume: 25 uL
    Amyloid A (Serum)
    (Serum Amyloid A - SAA)
    Suitable Sample:
    • PC (or clot activated tube)
    • Serum
    • Synovial fluid
    Unsuitable Sample:
    • LH
    • EDTA
    • FL
    • Samples from species other than equine or feline
    Minimum Sample Size:
    • 3 mL whole blood (plain/clot activated tube)
    • 1 mL serum
    • 0.5 mL synovial fluid in an EDTA tube
    Turnaround Time:
    • Test is run daily with results being reported the day of sample arrival

     

    Request/Test Included in:
    • SAA as a part of a biochemistry panel
      OR
    • SAA as a stand-alone test
    Comments:
    • SAA is an acute phase protein which is increased as part of the acute phase response.
    • It is increased in response to a wide range of processes which result in tissue injury; these include bacterial and viral infection, parasitic infestation, surgery, ischaemia, and neoplasia.
    • SAA is a major acute phase protein in the horse.
    • Following acute inflammation, SSA shows a rapid increase and peaks within 36-48 hours.
    • After the resolution of inflammatory disease, there is an equally rapid decrease.
    Internal Use Only:
    Internal Codes: B43
    Internal Laboratory: VP
    Internal Test Volume:
    ANA (Antinuclear Antibody)
    Suitable Sample:
    • PC (canine only)

     

    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 1 mL whole blood

     

    Turnaround Time:
    • Run Friday only
    • Cut off time 7pm previous working weekday
    Request/Test Included in:
    • ANA (canine)

     

    Comments:
    Internal Use Only:
    • Store in refrigerator

     

    Internal Codes: S01
    Internal Laboratory: VP
    Internal Test Volume:
    Anti-acetylcholine receptor antibody
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • EDTA
    • LH
    • CIT
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 3 - 4 weeks 
    Request/Test Included in:
    • Request test
      Comments:
      • Diagnostic test for myasthenia gravis
      • Can be used in dogs and cats
      Internal Use Only:
      • Allow at least two weeks as goes to the USA
      • Requires request form & TNT international con note (pack 4)
      • Quote VETPATH customer number 4432 when submitting samples
      Internal Codes: SU1
      Internal Laboratory: University of California, La Jolla, California USA
      http://medicine.ucsd.edu/vet_neuromuscular/forms.html
      Internal Test Volume: Website states that 1-2 mL of serum should be sent (NOT whole blood)
      Anti-Müllerian Hormone
      Suitable Sample:
      • PC
      Unsuitable Sample:
      • EDTA
      • LH
      Minimum Sample Size:
      • 2ml whole blood
      Turnaround Time:
      • 5 working days
      Request/Test Included in:
      • Request test
      Comments:
      • AMH is a hormone involved in gender differentiation in the developing embryo. In sexually mature animals it is produced by the granulosa cells of ovarian follicles and in the sertoli cells of the testicles. 
      • AMH levels markedly decline following neutering. 
      • Single measurement of AMH is therefore highly effective in differentiating between sexually intact and neutered dogs and cats of both sexes. AMH is also useful in identifying cases of ovarian remnant syndrome and cryptorchidism. 
      • The test is only suitable for animals over 6 months and repeat testing may be needed for animals between 6-12 months.
      • AMH can be used to detect granulosa cell tumours in mares (note that inhibin is no longer available) and used to distinguish between cryptorchid and castrated horses.
      • AMH concentration will tend to increase with sample storage.
      Internal Use Only:
      Internal Codes: E34
      Internal Laboratory: WDP
      Internal Test Volume: 300 - 500uL serum
      AP1 - Partial avian profile
      Suitable Sample:
      • LH
      • EDTA
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml
      Turnaround Time:
      • 4 - 6 hours
      Request/Test Included in:
      • Request test
      Comments:
      • AP1 includes CBC, AST, GLDH, glucose, total protein, albumin, globulin, calcium, phosphorus, uric acid.
      • Please list the priority tests for small samples. 

      Internal Use Only:
      Internal Codes: AP1
      Internal Laboratory: VP
      Internal Test Volume:
      AP2 - Complete avian profile
      Suitable Sample:
      • LH
      • EDTA
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml
      Turnaround Time:
      • 6 hours
      Request/Test Included in:
      • Request test
      Comments:
      • AP2 includes a CBC, AST, CK, GGT, ALP, CK, GGT, cholesterol, urea, uric acid, creatinine, total protein, albumin, globulin, glucose, calcium, phosphorus, Na, K, Cl.
      • Prioritise tests for small samples.
      Internal Use Only:
      Internal Codes: AP2
      Internal Laboratory: VP
      Internal Test Volume:
      Arsenic - acute exposure
      Suitable Sample:
      • LH
      • Urine
      Unsuitable Sample:
      • EDTA
      • PC
      Minimum Sample Size:
      • 2 mL of lithium heparin blood is the minimum, but 10 mL is preferred
      • 10 ml urine - unpreserved, just chilled
      Turnaround Time:
      •  Allow 10 working days

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Acute exposure = in the last few days
      • Samples must not be from a racing animal (written declaration is required). 
      • Test can be run in singlet (screening test) or quadruplicate (confirmation test).
      Internal Use Only:

      Cost to client: 

      Screen: $164.50 (single test)

      Confirmation: $554.50 (quadruplicate test)

      Internal Codes: X09 (other toxins, no specific code)
      Internal Laboratory: Chemistry Centre WA
      Internal Test Volume: 2 mL whole lithium heparin blood
      Arsenic - chronic exposure
      Suitable Sample:
      • Tissue - unpreserved

       

      Unsuitable Sample:
      • Blood

       

      Minimum Sample Size:
      • Minimum 0.1 gm digest weight
      • Preferred weight 2 gm
      Turnaround Time:
      •  Allow 7 working days

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Chronic exposure = 6 - 12 months
      • Sample must not come from a racing animal. A written declaration will be required.

       

      Internal Use Only:

      Cost to client: $229.50 + GST

      Internal Codes: X09 (other toxins, no specific code)
      Internal Laboratory: Chemistry Centre WA
      Internal Test Volume: N/A
      Aspergillus check (urine)
      Suitable Sample:
      • Urine (preferably cystocentesis sample)

       

      Unsuitable Sample:
      • Blood

       

      Minimum Sample Size:
      • 2 mL minimum
      • Preferably 10 mL to allow concentration
      Turnaround Time:
      • Initial report the first day
      • If negative, it is re-examined again 24 hours later
      Request/Test Included in:
      • Request test

       

      Comments:
      • Includes wet microscopy report and SG
      • Sediment is examined again after 24 hours for fungal hyphae in addition to the initial examination
      Internal Use Only: {Notes for Internal Use}
      Internal Codes: U10
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Aspergillus fumigatis Serology - nasal Aspergillosis
      Suitable Sample:
      • PC (non-haemolysed)

       

      Unsuitable Sample:
      • EDTA
      • LH
      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • 7 working days

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Aspergillus serology is primarily used for sino-nasal aspergillosis and is less sensitive for systemic aspergillosis. 
      • The test is specific for Aspergillus fumigatis and will not detect antibodies to other species.
      • A negative result does not rule out aspergillosis.
      • Consider testing galactomannan as a more effective method for screening for systemic Aspergillus infection.

       

      Internal Use Only:
      • QML
      Internal Codes: S07
      Internal Laboratory: QML
      Internal Test Volume: 400 uL serum
      Aspergillus Galactomannan - systemic Aspergillosis
      Suitable Sample:
      • PC
      Unsuitable Sample:
      • EDTA
      • LH
      Minimum Sample Size:
      • 2ml whole blood
      Turnaround Time:
      • 10 working days
      Request/Test Included in:
      • Request test
      Comments:
      • Galactomannan is a molecule found in the cell wall of Aspergillus sp and can be used as a screening test for systemic Aspergillosis.
      • Also consider checking a urine sample for fungal elements.
      • Serum should be frozen within 48 hours.
      Internal Use Only:

      Freeze if there more than a 24 hour delay in sending.

      Must have 500uL serum minimum

      Run Monday and Thursday - cut off is 11am.

      Internal Codes: G28
      Internal Laboratory: Pathwest (Fiona Stanley)
      Internal Test Volume: 500
      Aspirin
      Suitable Sample:
      • PC

       

      Unsuitable Sample:
      • EDTA

       

      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • 3 working days
      Request/Test Included in:
      • Request test
      Comments:
      • Paracetamol can also be done on this sample

       

      Internal Use Only: {Notes for Internal Use}
      Internal Codes:
      Internal Laboratory: Clinipath
      Internal Test Volume: 500 uL serum (absolute minimum is 100 uL serum, but can also do paracetamol on this)
      AST (Aspartate aminotransferase)
      Suitable Sample:
      • PC
      • LH
      Unsuitable Sample:
      • EDTA
      • FL
      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • 4 hours

       

      Request/Test Included in:
    • Request test
    • CP 2, 6
    • FP 2, 5
    • EP1, 2, 3, 4
    • RP1, 2, 3, 4
    • AP1, 2, 3, 4
    • Canine, feline & ruminant liver monitoring panels
    • Phenobarbitone monitoring panel
    • Comments:
      • AST may be from either skeletal muscle or liver origin
      • To assess muscle damage, measure both CK and AST
      • AST is also present in RBCs and activity may be higher in haemolysed samples
      Internal Use Only:
      Internal Codes:
      Internal Laboratory: VP
      Internal Test Volume: 30 uL
      Avian Sexing (DNA)
      Suitable Sample:
      • Feathers with some blood soaked in the quill (at least 2 feathers)
      • LH blood soaked into filter paper (200uL)
      Unsuitable Sample:
      • Mature chest feathers are unlikely to yield DNA for testing.
      • Feathers that have been collected from the ground or moulted feathers are not suitable.
      • Clipped feathers cannot be tested.
      Minimum Sample Size:
      Turnaround Time:
      • 2-3 weeks

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • One sample per container as it is important to avoid contamination.

       

      Internal Use Only:
      Internal Codes: G06
      Internal Laboratory: Gribbles
      Internal Test Volume: