Test Information

To locate information on a particular test, click on the relevant letter and follow the trail. eg To find information on sample requirements, test availability and turnaround time for FeLV, click on "F" and then click on the relevant test (FeLV soluble Ag or FeLV IFA).


A:G Ratio (albumin:globulin)
Suitable Sample:
  • PC
  • LH
  • EDTA
Unsuitable Sample:
  • CIT
  • FL 
Minimum Sample Size:
  • 2 mL whole blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
  • CP1, 2, 3, 4, 5, 6
  • FP1, 2, 3, 4, 5
  • EP1, 2, 3
  • RP1, 2, 3, 4
  • AP1, 2, 3, 4
Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
ACTH (Endogenous)
Suitable Sample:
  • EDTA plasma (plastic tube only)
  • There are published data for this assay method for dogs and horses, but not for other species
  • See Comments for specific collection and storage instructions
Unsuitable Sample:
  • PC
  • LH
  • SC
  • Samples from species other than canine or equine
Minimum Sample Size:
  • 1 ml whole blood
Turnaround Time:
  • The test is run Monday to Friday with a 2:00pm cut-off and on Saturday with a 1pm cut-off
  • Results available the same day
Request/Test Included in:
  • Request Test
Comments: The information below is also available on this website (ACTH)
  • Preferably fast the animal overnight prior to collection.
  • The animal should not be stressed or excited.
  • Collect a minimum of 1 mL of blood into a plastic EDTA tube.
  • Immediately place the sample in the refrigerator; this is important as ACTH deteriorates rapidly at room temperature.  Do not centrifuge the sample.
  • If possible, keep the sample cool during transit to the laboratory; this is particularly important during the summer months.
  • Samples collected and stored correctly are stable for at least 48 hours.
REF:Scott-Moncrieff JK, Koshko MA et al. (2003).Validation of a chemiluminescent enzyme immunometric assay for plasma adrenocorticotropic hormone in the dog. Vet Clin Path 23:180-187.
Internal Use Only:
  • Client Information sheet available (DCI-06)
  • Comment re dogs and horses ONLY added 28-5-08 (MMcC)
Internal Codes: E07
Internal Laboratory: VP
Internal Test Volume: 150 uL plasma
ACTH Stimulation Test (cortisol x 2)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • LH
  • EDTA
  • FL
Minimum Sample Size:
  • 2ml in each PC tube
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
Internal Use Only:
Internal Codes: E02
Internal Laboratory: VP
Internal Test Volume:
Adenovirus antibody titre
(Canine Adenovirus Antibody Titre - Infectious Canine Hepatitis)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 3 mL whole blood

 

Turnaround Time:
  • Referred test - Up to 4 weeks 
Request/Test Included in:
  • Request Test

 

Comments:
  • Samples are sent on Mondays and results are usually returned within two weeks.
  • Can be done as an individual test, or as a combination (distemper, parvovirus, adenovirus).
Internal Use Only:
  • Canine adenovirus (infectious canine hepatitis) testing is done at Biobest in Scotland.
  • Unless specifically requested, distemper and parvovirus antibody titres requested at the same time as an adenovirus titre are done at Vetpath and only the adenovirus test sent to Biobest.
Internal Codes:
  • S13B (adenovirus by VN)
  • S15B (combination distemper, parvovirus and adenovirus ONLY IF ALL are going to Biobest)
  • U
Internal Laboratory: Biobest, Scotland
Internal Test Volume: 1 ml clear serum
AGP
(no longer available)
Suitable Sample:
  • PC (serum) is preferred
  • Can be run on EDTA and LH plasma or other fluids if required (but these are not suitable for the other components of the FIP screen)
  • The test is feline specific
Unsuitable Sample:
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Monday to Friday on demand
  • Takes 24 hours to run
Request/Test Included in:
  • FIP Screen
  • Can be requested as an individual test
Comments:
  • Alpha-1 AGP is an acute phase protein that rises during inflammation. This test is not specific for FIP, but levels >1.5 g/L have a reported sensitivity of 85% and specificity of 100%, and efficiency of 90% in separating cats with FIP from cats with clinically similar conditions.

            REF: Vet Record (1997) 141:299-303.
Internal Use Only:
  • Agar gel immunodiffusion test which takes 24 hours to run
  • Feline specific
Internal Codes:
  • S28 for individual test
  • S23S for FIP Screen
  • S23SL for FIP Screen for laboratories
Internal Laboratory: VP
Internal Test Volume: Alpha-1 AGP alone 5 uL
Albumin
Suitable Sample:
  • PC
  • LH
  • EDTA
Unsuitable Sample:
  • FL

 

Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test
  • CP1, 2, 3, 4, 5, 6
  • FP1, 2, 3, 4, 5
  • EP1, 2, 3
  • RP1, 2, 3, 4
  • AP1, 2, 3, 4
  • Liver monitoring panels (all species)
Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
Aldosterone
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 7 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Published reference ranges available

 

Internal Use Only:
  • Added May 2008
  • Think the method is RIA (same as for published ranges but need to verify this with WDP)
Internal Codes: E31
Internal Laboratory:
  • WDP
  • Goes to WDP manual biochemistry
Internal Test Volume:
Allergy Test - Allercept IgE
Suitable Sample:
  • PC
  • Store at 2-8oC prior to sending to the laboratory
Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 6 mL whole blood (Take enough blood to yield at least 3ml of serum)
Turnaround Time:
  • 2-3 weeks

 

Request/Test Included in:
  • Allercept Allergy test (need to differentiate from the Canine ELISA)

 

Comments:
  • Store sample at 2-8oC before dispatch
  • Sample is to be sent to Vetpath as soon as possible
  • Can be used in dogs, cats and horses
Internal Use Only:
Internal Codes: S06
Internal Laboratory: Gribbles VIC
Internal Test Volume:
Allergy Test - Canine ELISA
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • SC
  • FL
  • Samples from species other than dogs
Minimum Sample Size:
  • 3.0 mL whole blood

 

Turnaround Time:
  • Run once a week
  • Contact laboratory for expected time of completion of test
Request/Test Included in:
  • Request Test

 

Comments:
  • Measures IgE to a wide range of potential allergens (specific allergens)
  • 48 Australian allergens are tested individually
  • Haemolysis interferes
  • Antiinflammatory doses of corticosteroids should not significantly suppress IgE production. However, immunosuppressive doses (or other immunosuppressants) will reduce Ab levels
  • Replaces the Greer test
Internal Use Only:
  • Run once a week and results are available within three days
  • Please contact the laboratory to confirm which day of the week the test is currently being run (may vary)
  • Test used to be called the Greer test, but is now called 48 Canine Allergy ELISA Test
Internal Codes: S05
Internal Laboratory: IDEXX Brisbane
Internal Test Volume: 1.0 mL non-haemolysed serum, absolute minimum 600µl.
ALP (Alkaline phosphatase)
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • CIT
  • FL
Minimum Sample Size:
  • 1.0 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • CP1, 2, 3, 4, 5, 6
  • FP1, 2, 3, 4, 5
  • EP1, 2, 3
  • AP2, 4
  • Liver monitoring panels (all species)
  • Phenobarbitone monitoring panel
Comments:
  • The corticosteroid isoenzyme is no longer measured.

 

Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
Alpha-1 Acid Glycoprotein
(no longer available)
Suitable Sample:
  • PC (serum) is preferred
  • Can be run on EDTA and LH plasma or other fluids if required (but these are not suitable for the other components of the FIP screen)
  • The test is feline specific
Unsuitable Sample:  
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Monday to Friday on demand
  • Takes 24 hours to run
Request/Test Included in:
  • FIP Screen
  • Can be requested as an individual test
Comments:
  • Alpha-1 AGP is an acute phase protein that rises during inflammation.  This test is not specific for FIP, but levels >1.5 g/L have a reported sensitivity of 85% and specificity of 100%,  and efficiency of 90% in separating cats with FIP from cats with clinically similar conditions.

            REF: Vet Record (1997) 141:299-303.
Internal Use Only:
  • Agar gel immunodiffusion test which takes 24 hours to run
  • Feline specific
Internal Codes:
  • S28 for individual test
  • S23S for FIP Screen
  • S23SL for FIP Screen for laboratories
Internal Laboratory: VP
Internal Test Volume: Alpha-1 AGP alone  5 uL
ALT (Alanine aminotranferase)
Suitable Sample:
  • PC
  • LH
  • EDTA
Unsuitable Sample:
  • FL

 

Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test
  • CP1, 2, 3, 4, 5, 6
  • FP1, 2, 3, 4, 5
  • Canine & feline liver monitoring panels
  • Phenobarbitone monitoring panel
Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 30 uL
Amylase
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • FL
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test
  • CP1, 2, 5, 6
Comments:
Internal Use Only: Used to be able to use EDTA plasma, cannot with current method.
Internal Codes: {Codes(s)}
Internal Laboratory: VP
Internal Test Volume: 25 uL
Amyloid A (Serum)
(Serum Amyloid A - SAA)
Suitable Sample:
  • PC (or clot activated tube)
  • Serum
  • Synovial fluid
Unsuitable Sample:
  • LH
  • EDTA
  • FL
  • Samples from species other than equine or feline
Minimum Sample Size:
  • 3 mL whole blood (plain/clot activated tube)
  • 1 mL serum
  • 0.5 mL synovial fluid in an EDTA tube
Turnaround Time:
  • Test is run daily with results being reported the day of sample arrival

 

Request/Test Included in:
  • SAA as a part of a biochemistry panel
    OR
  • SAA as a stand-alone test
Comments:
  • SAA is an acute phase protein which is increased as part of the acute phase response.
  • It is increased in response to a wide range of processes which result in tissue injury; these include bacterial and viral infection, parasitic infestation, surgery, ischaemia, and neoplasia.
  • SAA is a major acute phase protein in the horse.
  • Following acute inflammation, SSA shows a rapid increase and peaks within 36-48 hours.
  • After the resolution of inflammatory disease, there is an equally rapid decrease.
Internal Use Only:
Internal Codes: B43
Internal Laboratory: VP
Internal Test Volume:
ANA (Antinuclear Antibody)
Suitable Sample:
  • PC (canine only)

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Friday only
  • Cut off time 7pm previous working weekday
Request/Test Included in:
  • ANA (canine)

 

Comments:
Internal Use Only:
  • Store in refrigerator

 

Internal Codes: S01
Internal Laboratory: VP
Internal Test Volume:
Anaplasma platys (previously called Ehrlichia platys)
Suitable Sample:
  • EDTA

 

Unsuitable Sample:
  • PC
  • LH
  • FL
Minimum Sample Size:
  • 2ml whole blood
Turnaround Time:
  • Weeks (see comment)

 

Request/Test Included in:
  • Request test
Comments:
  • Contact Vetpath to check on availability of this test.

 

Internal Use Only:
  • Research basis only at this time (see Peter Irwin). Thrombocytopenic samples are accumulated and then batched for PCR. Not available on a commercial basis at this time.
  • From 03/06 Ryan at Murdoch is not able to do this testing, but check with J Jardine.
Internal Codes:
Internal Laboratory: Murdoch University
Internal Test Volume:
Anti-acetylcholine receptor antibody
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 3 - 4 weeks 
Request/Test Included in:
  • Request test
    Comments:
    • Diagnostic test for myasthenia gravis
    • Can be used in dogs and cats
    Internal Use Only:
    • Allow at least two weeks as goes to the USA
    • Requires request form & TNT international con note (pack 4)
    • Quote VETPATH customer number 4432 when submitting samples
    Internal Codes: SU1
    Internal Laboratory: University of California, La Jolla, California USA
    http://medicine.ucsd.edu/vet_neuromuscular/forms.html
    Internal Test Volume: Website states that 1-2 mL of serum should be sent (NOT whole blood)
    Anti-Müllerian Hormone
    Suitable Sample:
    • PC
    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 2ml whole blood
    Turnaround Time:
    • 5 working days
    Request/Test Included in:
    • Request test
    Comments:
    • AMH is a hormone involved in gender differentiation in the developing embryo. In sexually mature dogs it is produced by the granulosa cells of ovarian follicles and in the stertoli cells of the testicles. 
    • AMH levels markedly decline following neutering. 
    • Single measurement of AMH is therefore highly effective in differentiating between sexually intact and neutered dogs and cats of both sexes. AMH is also useful in identifying cases of ovarian remnant syndrome and cryptorchidism. 
    • The test is only suitable for animals over 6 months and repeat testing may be needed for animals between 6-12 months.
    • AMH can be used to detect granulosa cell tumours in mares (note that inhibin is no longer available) and used to distinguish between cryptrorchid and castrated horses.
    • AMH concentration will tend to increase with sample storage.
    Internal Use Only:
    Internal Codes: E34
    Internal Laboratory: WDP
    Internal Test Volume:
    AP1 - Partial avian profile
    Suitable Sample:
    • LH
    • EDTA
    • PC
    Unsuitable Sample:
    Minimum Sample Size:
    • 1ml
    Turnaround Time:
    • 4 - 6 hours
    Request/Test Included in:
    • Request test
    Comments:
    • AP1 includes CBC, AST, GLDH, glucose, total protein, albumin, globulin, calcium, phosphorus, uric acid.
    • Please list the priority tests for small samples. 

    Internal Use Only:
    Internal Codes: AP1
    Internal Laboratory: VP
    Internal Test Volume:
    AP2 - Complete avian profile
    Suitable Sample:
    • LH
    • EDTA
    • PC
    Unsuitable Sample:
    Minimum Sample Size:
    • 1ml
    Turnaround Time:
    • 6 hours
    Request/Test Included in:
    • Request test
    Comments:
    • AP2 includes a CBC, AST, CK, GGT, ALP, CK, GGT, cholesterol, urea, uric acid, creatinine, total protein, albumin, globulin, glucose, calcium, phosphorus, Na, K, Cl.
    • Prioritise tests for small samples.
    Internal Use Only:
    Internal Codes: AP2
    Internal Laboratory: VP
    Internal Test Volume:
    Arsenic - acute exposure
    Suitable Sample:
    • LH
    • Urine
    Unsuitable Sample:
    • EDTA
    • PC
    Minimum Sample Size:
    • 2 mL of lithium heparin blood is the minimum, but 10 mL is preferred
    • 10 ml urine - unpreserved, just chilled
    Turnaround Time:
    •  Allow 7 working days

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • Acute exposure = in the last few days

     

    Internal Use Only: Cost as of 29-03-2010 is $220 plus GST, but need to check with the lab.
    Internal Codes: X09 (other toxins, no specific code)
    Internal Laboratory: Chemistry Centre WA
    Internal Test Volume: 2 mL whole lithium heparin blood
    Arsenic - chronic exposure
    Suitable Sample:
    • Tissue - unpreserved

     

    Unsuitable Sample:
    • Blood

     

    Minimum Sample Size:
    • Minimum 0.1 gm digest weight
    • Preferred weight 2 gm
    Turnaround Time:
    •  Allow 7 working days

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • Chronic exposure = 6 - 12 months

     

    Internal Use Only: {Notes for Internal Use}
    Internal Codes: X09 (other toxins, no specific code)
    Internal Laboratory: Chemistry Centre WA
    Internal Test Volume: N/A
    Aspergillus check (urine)
    Suitable Sample:
    • Urine (preferably cystocentesis sample)

     

    Unsuitable Sample:
    • Blood

     

    Minimum Sample Size:
    • 2 mL minimum
    • Preferably 10 mL to allow concentration
    Turnaround Time:
    • Initial report the first day
    • If negative, it is re-examined again 24 hours later
    Request/Test Included in:
    • Request test

     

    Comments:
    • Includes wet microscopy report and SG
    • Sediment is examined again after 24 hours for fungal hyphae in addition to the initial examination
    Internal Use Only: {Notes for Internal Use}
    Internal Codes: U10
    Internal Laboratory: VP
    Internal Test Volume: N/A
    Aspergillus PCR
    Suitable Sample:
    • EDTA blood
    Unsuitable Sample:
    • Serum
    • Urine
    Minimum Sample Size:
    • 2ml whole blood
    Turnaround Time:
    • 5 working weekdays
    Request/Test Included in:
    • Request test
    Comments:
    • Blood must be collected aseptically as environmental contaminants can affect the test.
    Internal Use Only:
    • Referral lab - Pathwest PCR QEII Medical centre Nedlands

     

    Internal Codes: G17
    Internal Laboratory: Pathwest QEII Nedlands
    Internal Test Volume:
    Aspergillus Serology
    Suitable Sample:
    • PC (non-haemolysed)

     

    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 4 mL whole blood

     

    Turnaround Time:
    • 7 working days

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • A negative result does not rule out aspergillosis.
    • Consider testing galactomannan as a more effective method for screening for Aspergillus infection.

     

    Internal Use Only:
    • Set up twice a week (updated 28/7/2011)
    • Sent to PathWest, if no reagent there sent to Queensland
    • Some hemolysis tolerated
    Internal Codes: S07
    Internal Laboratory:
    • PathWest preferably
    • QML (Symbion) Queensland 07 3121 4444 if PathWest can't do
    Internal Test Volume: 10 uL serum but prefer 0.5ml minimum.
    Aspirin
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • EDTA

     

    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 3 working days
    Request/Test Included in:
    • Request test
    Comments:
    • Paracetamol can also be done on this sample

     

    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    Internal Laboratory: Clinipath
    Internal Test Volume: 500 uL serum (absolute minimum is 100 uL serum, but can also do paracetamol on this)
    AST (Aspartate aminotransferase)
    Suitable Sample:
    • PC
    • LH
    Unsuitable Sample:
    • EDTA
    • FL
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
  • Request test
  • CP 2, 6
  • FP 2, 5
  • EP1, 2, 3, 4
  • RP1, 2, 3, 4
  • AP1, 2, 3, 4
  • Canine, feline & ruminant liver monitoring panels
  • Phenobarbitone monitoring panel
  • Comments:
    • AST may be from either skeletal muscle or liver origin
    • To assess muscle damage, measure both CK and AST
    • AST is also present in RBCs and activity may be higher in haemolysed samples
    Internal Use Only:
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume: 30 uL
    Avian Sexing (DNA)
    Suitable Sample:
    • Either blood or feathers may be submitted, but blood is the preferred sample.
    • Blood: Heparinised blood is required. From small birds, a few drops is sufficient.
    • Feathers
    Unsuitable Sample:
    • Mature chest feathers are unlikely to yield DNA for testing.
    • Feathers that have been collected from the ground or moulted feathers are not suitable.
    • Clipped feathers cannot be tested.
    Minimum Sample Size:
    Turnaround Time:
    • 2-3 weeks

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • One sample per container as it is important to avoid contamination.

     

    Internal Use Only:
    • Samples going to Biobest, can be done on feathers or LH blood, but prefer LH blood.
    • No longer being sent to DNA Solutions for sex determination (20/3/07)
    Internal Codes: G06
    Internal Laboratory: BioBest, UK
    Internal Test Volume: