Suitable Sample: |
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Unsuitable Sample: |
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Minimum Sample Size: |
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Turnaround Time: |
- Run on Wednesday
- Cut off is 7pm the previous working day
- Results are reported by 2pm the next day
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Request/Test Included in: |
- Individual test
- Combined feline herpesvirus, calicivirus and panleukopenia
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Comments: |
This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
- Cats with a titre of <1:5 may not be adequately protected and revaccination should be considered.
- Cats with a titre of 1:5 but <1:20 may consider revaccination.
- Cats with a titre of 1:20 or greater are considered protected and may not need revaccination. These animals should have their titre rechecked in approximately one year.
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Internal Use Only: |
- Store 2-8oC
- Serial dilutions are preformed from 1:5 with four-fold up to 1:80 (greater dilutions may be requested in some cases, charges are negotiable).
- If the test is being done to detect exposure to calicivirus, acute specimens should be drawn at the start of illness and convalescent specimens should be obtained at 2 to 4 week intervals to check titre changes.
- Samples can still be sent to Biobest in Scotland if the client requests this.
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Internal Codes: |
- S27F
- S27L for laboratories
- S24F (combination calici, herpes, panleukopenia)
- S24L (combination calici, herpes, panleu)
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Internal Laboratory: |
VP |
Internal Test Volume: |
- 50-100 uL serum for single tests
- approximately 200 uL of serum for the combined tests
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Suitable Sample: |
- PC
- If the sample is the first of a pair, please indicate this on the submission form so that the sample can be held and the pair processed together.
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Unsuitable Sample: |
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Minimum Sample Size: |
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Turnaround Time: |
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Request/Test Included in: |
- Request test
- It is essential to indicate if this is a single sample, or the first/second of a pair. All paired samples must be processed at the same time, even if the first of the pair has already been processed (ie it would need to be repeated)
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Comments: |
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For detection of of a rise in Ab (indicative of current infection) paired sera (acute and convalescent) are required 14 days apart.
- Paired samples must be processed at the same time; the first of the pair needs to be separated and frozen until the second sample arrives. If the first of the pair has already been processed, it will need to re-tested with the second sample (ie it would need to be run, and charged for, twice).
- Store whole blood at 4oC, but if transport is delayed it is necessary to separate the serum/plasma from the cells.
- Serum/plasma can be frozen at -20oC or -70oC (do not freeze whole blood).
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Internal Use Only: |
- Now goes to the Ag Dept NOT Uni of Melbourne
- Send separated if the sample will not be sent within 36 hours.
- Transport cool (on ice).
- Can be frozen.
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Internal Codes: |
SV2 (single) SV3 (paired) |
Internal Laboratory: |
DPIRD |
Internal Test Volume: |
4ml whole blood |