| Suitable Sample: |
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| Unsuitable Sample: |
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| Minimum Sample Size: |
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| Turnaround Time: |
- Run on Wednesday
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- Cut off is 7pm the previous working day
- Results are reported by 2pm the next day
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| Request/Test Included in: |
- CDV antibody titres by IFA
- Combined CDV & CPV IFA titre (sample requirements are the same as for CDV alone, both tests run the same day)
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| Comments: |
Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.
Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.
INTERPRETATION:
- Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
- Titres of >1:5 are considered seropositive.
- Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
- Dogs with titres of >1:20 probably do not require revaccination.
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| Internal Use Only: |
- Original file 21-07-2004
- Serial dilutions are performed from 1:5 with four fold diltions to 1:80 (greater dilutions may be requested in some instances - charges are negotiable.
- Samples can still be sent to Biobest in Scotland; this is done only if the client specifically requests it.
- Store samples at 2-8oC
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| Internal Codes: |
- S12F (CDV by IFA)
- S14F (combination CDV & CPV by IFA, no adenovirus)
- S12L (CDV by IFA for laboratory)
- S14L (combination CDV and CPV for laboratory)
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| Internal Laboratory: |
VP |
| Internal Test Volume: |
100 uL to 200 uL is needed per test |
| Suitable Sample: |
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| Unsuitable Sample: |
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| Minimum Sample Size: |
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| Turnaround Time: |
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| Request/Test Included in: |
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| Comments: |
- Referred test
- Distemper serology can also be done by IFA at Vetpath
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| Internal Use Only: |
- If only canine distemper serology is required, use IFA
- The VN (virus neutralisation) test is used only when specifically requested by the client
- Samples are sent on Mondays and results are returned within two weeks.
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| Internal Codes: |
- S12B Distemper Ab by VN (virus neutralisation)
- S15B Combination distemper, parvo and adenovirus all done at Biobest
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| Internal Laboratory: |
Biobest, Scotland |
| Internal Test Volume: |
1 ml clear serum |